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+919820112338General Information of Bile Extract
It is mainly used to add flavor.
Bile salt constitutes pure substances, sodium conjugates of glycotaurocholate, and those are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics. Bile inhibits Enterococcus and Staphylococcus species.ÃÂ
Specification:-
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Physical and Chemical parameter-
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S.No. |
Parameters |
Specifications |
1. |
Appearance |
Greenish to yellowish colour free flowing powder, no pungent smell. |
2. |
Solubility (2% soln. at 25oC) |
Soluble in distilled water and alcohol. |
3. |
Clarity (2% Soln. at 121ÃÂÃÂâÃÂÃÂÃÂÃÂÃÂúC.)* |
Clear solution. No ppt. |
4. |
pHà(2% Soln. at 25ÃÂÃÂâÃÂÃÂÃÂÃÂÃÂúC) |
6.5 - 8.0 |
5. |
Loss on drying ( at 105ÃÂÃÂâÃÂÃÂÃÂÃÂÃÂúC) |
NMT - 5.0% |
6. |
Total Ash |
NMT - 20.0% |
7. |
Cholic Acid (as Sodium Cholate) |
NLT - 45.0% |
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Microbiological parameter-
S.No. |
Parameters |
Specifications |
1. |
Total viable count |
NMT- 10,000cfu/gm |
2. |
Escherichia coli |
Negative |
3. |
Salmonella spp. |
Negative |
4. |
Staphylococcus aureus |
Negative |
5. |
Yeast & molds |
100/gm |
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Growth promotion test-
Cultural characteristic observed in MacConkey agar with 0.5% Bile salt powder after incubation at 35-37oC.
S.No. |
Microorganism |
ATCC |
GROWTH |
Recovery |
1. |
Staphylococcus aureus |
25923 |
Inhibited |
----------- |
2. |
Escherichia coli |
25922 |
Luxuriant / bile ppt. |
>= 50% |
3. |
Enterococcus faecalis |
29212 |
Fair-Good |
>= 50% |
4. |
Salmonella typhimurium |
14028 |
Luxuriant |
>= 50% |
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