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Tryptone is an array of peptides formed as protease trypsin digests casein. It is used in microbiology for producing Iysogeny broth for the E. coli and other microorganisms growth. It is an amino acids source for the growth of bacteria.
Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. Tryptone is commonly used in microbiology to produce lysogeny broth for the growth of E. coli and other microorganisms. It provides a source of amino acids for the growing bacteria.
Applications: BIOLOGICAL CULTURE MEDIA
Specification:-
Product Parameters | Specification |
Appearance | Off white to creamish yellow colour, free flowing powder having characteristic odour but not pungent smell. |
Solubility (2% Soln. at 25 degree centigrade) | Soluble in distilled Water, Clear, Insoluble in alcohol. |
Clarity (2% Soln. at 121degree centigrade) | Clear solution. No ppt. |
Product Parameters | Specification |
pH (2% Soln. at 25 degree centigrade) | 6.5-7.5 |
Loss on drying (at 105 degree centigrade) | NMT- 5.0% |
Total Nitrogen ( DWB) | NLT- 12.0% |
Amino Nitrogen | NLT- 2.0% |
Total Ash | NMT- 10.0% |
Chloride (as NaCl) | NMT- 5.0% |
Indole Test | Positive |
Product Parameters | Specification |
Total viable count | NMT- 10,000cfu/gm |
Escherichia coli | Negative |
Salmonella spp. | Negative |
Staphylococcus aureus | Negative |
Yeast and molds | 100/gm |
Growth Promotion Test
Cultural Characteristic observed in 2% Tryptone and 1.5% agar after incubation at 35-37 degree centigrade for 18-24 hours.
MICROORGANISMS | ATCC | GROWTH |
Staphylococcus aureus | 25923 | Good-Luxuriant |
Escherichia coli | 25922 | Good-Luxuriant |
Pseudomonas aeruginosa | 27853 | Good-Luxuriant |
Bacillus subtilis | 6633 | Good-Luxuriant |
Enterococcus faecalis | 29212 | Good-Luxuriant |
Streptococcus pyogenes | 19615 | Good-Luxuriant |